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TT.I.D

sveglia 11:00 - 12:30

Cells on Chip: Boosting Cellular Measurements
for advanced pharmacological and clinical applications
SYNOPSIS
TT.I.D.1 Cristina COLOSI
Center for Life and Nanoscience – Italian Institute of Technology, Rome
Microfluidic Bioprinting for the creation of human-derived in vitro 3D cellular models  abst
TT.I.D.2 Fabrizio MATTEI
National Institute of Health, Rome
Exploiting Microfluidic chips to investigate cancer and immune cells crosstalk abst
TT.I.D.3 Federica CASELLI
University of Rome Tor Vergata
Single-cell impedance spectroscopy for label-free diagnosticsa  abst
TT.I.D.4
Alberto RAINER
Tissue Engineering Lab - University Campus Bio-Medico, Rome
Microscopy and hyperspectral imaging for cell-on-a-chip applications  abst
Chair: Luca BUSINARO, CNR-IFN, Rome
In collaboration with: CNR - IFN

SYNOPSIS

 

Considerable efforts have been recently devoted to the development of advanced micro-systems for studying cellular functions.  Advantages such as high throughput analysis, reduction of cell population heterogeneity, reproducibility and well-defined cell culture conditions make these systems very attractive for a wide range of applications ranging from biomedical research and drug discovery to toxicology, stem cell research and therapy. In this regard, array-based formats have proved their efficacy as enhanced-throughput platforms for 3D culture of various cell types, and much effort is now being directed towards cell culture models that better reflect in vivo function. In this context, organs-on-chip have emerged to provide new tools for better evaluating the effects of various chemicals on human tissues.

During the session, thus, we will introduce different aspects of cell measurements through microfluidic chips, from single cell analysis, through impedance spectroscopy, to immune system interaction and observation. This will be done considering also different fabrication approaches: 3D printing of cell loaded gels, and standard top-down approach through soft lithography. Cells on chip means also to gain full access to modern microscopy  to measure phenomena previously observable only in invivo measurements, thus an overview of the meaning of bringing complex biological process under the microscope will be addressed.


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